文獻引用產品:
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產品貨號
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產品名稱
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CAS
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規格
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B21634
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蕓香柚皮苷
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14259-46-2
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分析標準品，HPLC≥98%
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摘要：Acetaminophen (APAP) overdose is the predominant cause of drug-induced liver injury worldwide. The macroautophagy/autophagy-lysosomal pathway (ALP) is involved in the APAP hepatotoxicity. TFEB (transcription factor EB) promotes the expression of genes related to autophagy and lysosomal biogenesis, thus, pharmacological activation of TFEB-mediated ALP may be an effective therapeutic approach for treating APAP-induced liver injury. We aimed to reveal the effects of narirutin (NR), the main bioactive constituents isolated from citrus peels, on APAP hepatotoxicity and to explore its underlying mechanism. Administration of NR enhanced activities of antioxidant enzymes, improved mitochondrial dysfunction and alleviated liver injury in APAP-treated mice, whereas NR did not affect APAP metabolism and MAPK/JNK activation. NR enhanced TFEB transcriptional activity and activated ALP in an MTOR complex 1 (MTORC1)-independent but PPP3/calcineurin-dependent manner. Moreover, knockout of Tfeb or knockdown of PPP3CB/CNA2 (protein phosphatase 3, catalytic subunit, beta isoform) in the liver abolished the beneficial effects of NR on APAP overdose. Mechanistically, NR bound to PPP3CB via PRO31, LYS61 and PRO347 residues and enhanced PPP3/calcineurin activity, thereby eliciting dephosphorylation of TFEB and promoting ALP, which alleviated APAP-induced oxidative stress and liver injury. Together, NR protects against APAP-induced liver injury by activating a PPP3/calcineurin-TFEB-ALP axis, indicating NR may be a potential agent for treating APAP overdose.
文獻鏈接：https://www.tandfonline.com/doi/abs/10.1080/15548627.2023.2179781
摘要：Oligosaccharides are recognized as prebiotics. The structure of oligosaccharides is closely related to bioactivity. However, how to precisely and quickly identify the structure remains a bottleneck. In this work, ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed to analyze the structure of oligosaccharides. Oligosaccharides mainly presented as sodium adduct in the total ion chromagram. By analyzing the parent ions and daughter ions, the structure of oligosaccharides could be identified. The fragmentation patterns of oligosaccharides were summarized for various glycosidic linkages. This protocol showed advantages when comparing with MALDI-TOF-MS/MS technqiue. A mixed oligosaccharides (maltodextrins) were analyzed, and the oligosaccharides composition was completely revealed. These results suggested that UPLC-MS/MS procotol was effective to identify oligosaccharides structure, and could be applied in identification of polysaccharide structure.
文獻鏈接：https://www.sciencedirect.com/science/article/pii/S0268005X23001042
摘要：This study explored the effects of wheat bran arabinoxylan (AX) of various molecular structures on the functional and in vitro digestibility of wheat starch. AX was separated with sequential ethanol precipitation into different fractions (L-Fra and H-Fra), followed by acidic treatment at varied time to convert L-Fra into different subfractions (H-Sub, M-Sub and L-Sub), and the structural features of AX and its subsequent fractions were compared. The Mw and branching degree of the fractions were decreased in the order of H-Fra (545 kDa, 1.15), L-Fra (86 kDa, 0.35), H-Sub (38 kDa, 0.16), M-Sub (21 kDa, 0.10) and L-Sub (7 kDa, 0.08). The functional properties, such as pasting properties, rheological properties, thermal properties, and their effect on the in vitro digestibility of wheat starch-AX combinations, were closely related to the molecular structures of AX. AX with higher Mw, higher branching degree and phenolic content could better inhibit the starch gelatinization and retrogradation, thus reducing the apparent viscosity and gelling properties of wheat starch. The possible mechanisms can be illustrated as 1) AX could compete for water with starch to inhibit granule expansion; 2) AX could not only adsorb on the surface of starch granules through hydrogen bonds but also entangle with the leached amylose and wrap on the surface of granules; 3) AX contained phenols, leading them to inhibit granule expansion through non-covalent interactions with starch molecules. Overall, this study provides a theoretical basis to improve the quality of wheat starch foods and extend the shelf life of wheat foods.
文獻鏈接：https://www.sciencedirect.com/science/article/pii/S0268005X23001613