CY (Cyanine) 熒光染料, 也叫菁染料，是一類具有多聚次甲基共軛鏈接的合成熒光染料。吸光度和熒光波長可通過選擇聚次甲基橋長來控制。CY染料由于其特異性高、毒性小等優(yōu)點，在熒光染料界素有染料“交際花”的美譽，經(jīng)常被用于細(xì)胞顯影，生物篩選，蛋白免疫印跡，生物醫(yī)學(xué)顯影，小動物體內(nèi)成像等。
| 產(chǎn)品貨號 |
產(chǎn)品名稱 |
CAS |
規(guī)格 |
| S81148 |
Cy7 |
943298-08-6 |
96% |
摘要：The One-Bead One-Compound (OBOC) library screening is an efficient technique for identifying targeting peptides. However, due to the relatively large bead size, it is challenging for the OBOC method to be applied for in vivo screening. Herein, we report an in vivo Localized Instillation Beads library (LIB) screening method to discover targeting peptides with the OBOC technique. Inspired by localized instillation, we constructed a cavity inside of a transplanted tumor of a mouse. Then, the OBOC heptapeptide library was injected and incubated inside the tumor cavity. After an efficient elution process, the retained beads were gathered, from which three MDA-MB-231 tumor-targeting heptapeptides were discovered. It was verified that the best peptide had 1.9-fold higher tumor accumulation than the commonly used targeting peptide RGD in vivo. Finally, two targeting proteins were discovered as potential targets of our targeting peptide to the MDA-MB-231 tumor. The in vivo LIB screening method expands the scope of OBOC peptide screening applications to discover targeting peptides in vivo feasibly and reliably.
文獻(xiàn)鏈接：https://pubs.acs.org/doi/abs/10.1021/acsnano.2c09894
| 產(chǎn)品貨號 |
產(chǎn)品名稱 |
CAS |
規(guī)格 |
| S25173 |
Cy5 |
1032678-07-1 |
95%
|
摘要：The stomach acid degradation, mucus clearance and intestinal epithelial impermeability severely limit the oral delivery of polypeptide drugs. To simultaneously address the three major barriers, novel self-assembled core-shell nanosystems (CA-NPs) were designed. The fabricated shell of citric acid cross-linked carboxymethyl cellulose (CA-CMC) wrapped on core nanoparticles (HA-NPs) maintained the integrity of CA-NPs in the stomach. When CA-NPs passed through the stomach, the CA-CMC shell was gradually degraded to release the core HA-NPs in the intestine. HA-NPs with numerous hydrophilic groups and mannose side chains rapidly penetrated through the mucus layer and efficiently transcellular transported via the glucose transporter (GLUT)-mediated and paracellular transport through reversible opening of tight junctions (TJs) by CA-CMC. The oral bioavailability and therapeutic effects of CA-NPs-loaded polypeptide colistin against Escherichia coli (E. coli) bacteremia in mice were significantly increased compared with the native colistin, respectively. Good safety was observed following oral daily delivery for 14 consecutive days. Thus, CA-NPs may offer a promising strategy for the oral delivery of polypeptide drugs.
文獻(xiàn)鏈接：https://www.sciencedirect.com/science/article/abs/pii/S0168365922007015